One of the vital fascinating observations and one among the best in vitro experiments that indicates these reverse results of sterols and fatty acids is their influence upon the oxidation processes in which purple cells intervene. When a sample of unusual venous blood is treated with a preparation of cholesterol or nonsaponifiable fractions, utilizing the above mentioned approach and after its separation from the cholesterol it is agitated with air or oxygen which is passed by means of these samples, the shade becomes a brilliant vermilion purple, and this persists for a long time. When the identical venous blood is handled with a preparation of polyunsaturated fatty acids as mentioned above, the colour becomes very darkish, almost black purple. When air or oxygen is handed by these samples, the blood turns into lighter in color for only a short time, the darker colour reappearing inside a few minutes. One is immediately impressed by the similarity of the cholesterol treated blood to arterial blood, while the fatty acid treated blood is much like venous blood, and particularly to the coloration of venous blood in instances of shock.
We tried to tie in these findings with the commentary of Binet concerning the modifications in blood fatty acids when passing by means of the lungs. He has been in a position to indicate that the quantity of the polyunsaturated members appears to be lowered by the passage of blood through the lungs. We could present that the red cells leaving the pulmonary vascular bed are somewhat richer within the unbound cholesterol than they have been in the blood which entered the lungs. The lipid content is altered in an opposite means because the blood travels by means of the general circulation. That's, the polyunsaturated fatty acid content material is elevated in the pink cells whereas the quantity of free cholesterol seems to be diminished. The sterol richer crimson cells seem capable of retaining for a longer time period, the amount of oxygen which hemoglobin has mounted, whereas a speedy discount of oxyhemoglobin is seen within the crimson cells when the polyunsaturated fatty acids intervene.
This led us to consider an intervention of those two groups of lipids in relation to the oxygen transportation by the red cells. Bearing in mind the fact that whereas cholesterol reduces cell permeability and polyunsaturated fatty acids enhance it, an alternating intervention of those lipids seems to play a job in a better distribution of oxygen. The oxygen which is fixed by hemoglobin when the pink cells have handed the lungs, is essentially retained as such by the intervention of the sterols until they attain the purpose in the tissues the place liberation of oxygen is important, this being favored now by the intervention of the fatty acids. The abnormally dark coloration of the blood resulting from its therapy in vitro with polyunsaturated fatty acids has steered the intervention of such substances in those clinical circumstances by which comparable color modifications are noted within the blood as in shock. We will present our studies in shock under. For the moment we are going to solely word that within the state of shock experimentally induced by trauma, burns or irradiation, BloodVitals SPO2 or BloodVitals home monitor found in terminally ailing adrenalectomized animals, these animals haven't only a high fatty acid content, but that the form of fatty acids encountered are usually not the identical as in regular animals.
We have mentioned these abnormal fatty acids above. The existing differences have been shown by measuring the quantity of oxalic acid that's produced when these fatty acids are submitted to a cautious standardized oxidative fission. The oxidative fission of the fatty acids not solely from their complete body but even from their blood has proven that for normal animals, no oxalic acid may very well be found, leading to the assumption that no conjugated fatty acids are present. However, oxalic acid appeared when fatty acids obtained from animals in shock or BloodVitals home monitor from their blood have been damaged down with the analytical methodology utilized. Of specific significance for the pathogenic role of those fatty acids is the very fact which we'll talk about once more below, that dying appears to ensue when the conjugation of fatty acids reaches a sure worth, which is roughly the identical whether or not the animal has been traumatized, burned, irradiated or adrenalectomized, and impartial of the truth that loss of life happens in a short time or several days.